dc generation medium a Search Results


culture medium  (PromoCell)
94
PromoCell culture medium
Culture Medium, supplied by PromoCell, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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mo dc differentiation medium  (Miltenyi Biotec)
95
Miltenyi Biotec mo dc differentiation medium
Mo Dc Differentiation Medium, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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dc generation medium xf  (PromoCell)
94
PromoCell dc generation medium xf
Dc Generation Medium Xf, supplied by PromoCell, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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aim-v  (Thermo Fisher)
90
Thermo Fisher aim-v
Aim V, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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dc generation medium a  (PromoCell)
93
PromoCell dc generation medium a
Mechanisms of DC-mediated cross-presentation of LA. a Pathway diagram of DC cross-presenting epitopes. b IFN-γ secretion by ELISPOT assay ( n = 3). c–f Monocytes were obtained with a monocyte attachment medium (C-28051, PromoCell) from PBMCs and then cultured in DC generation <t>medium</t> <t>A</t> <t>(C21050,</t> PromoCell) to harvest immature DCs. Immature DCs were treated with 50 μg/ml peptide twice with an interval of 24 h. Maturation of YL/LA-pulsed DCs was promoted ( n = 3). c Morphological examination. d Presentation ability. e Uptake capacity. f IL-12p70 secretion by ELISA. g LA was mainly taken up by immature DCs through an active transport (37 °C), and others might directly bind to DCs (4 °C). h LA was mainly taken up by phagocytosis (inhibited with cytochalasin D), partly by receptor-mediated internalization (inhibited with heparin) ( n = 3). i Immunofluorescence analysis of co-localization between LA (green, FITC) and different subcellular compartments (red, early endosome, lysosome or endoplasmic reticulum) in immature DCs (blue, DAPI, nuclear) ( n = 3). j The trace of LA was mainly concentrated on MHC-I molecular internalization (inhibited with primaquine), rather than vesicle secretion (inhibited with brefeldin A) and TAP transport (inhibited with ICP47) in DCs ( n = 3). k The combination of LA-FITC and HLA-A2.1 on DC surface (blue, DAPI, nuclear; red, Dil, membrane; green, LA-FITC). l The presentation of LA (963.6) or YL (912.3) on DC surface by UPLC-QTOF-MS. m Immunoblot analysis of total Zap70 proteins and phosphorylated (p-) Zap70 in whole-cell lysates of epitope/DC-CTLs ( n = 3). NC, negative control, water. NP, negative peptide, the peptide (PPGRPSPDN) derived from ECM1 with largest predicted IC 50 of affinity with HLA-A2.1. d–f , h , j P values were obtained from independent-samples t-test; error bars denote standard deviation (SD). d–f Compared with NC, * P < 0.05, ** P < 0.01; h Compared with 37 °C blank, * P < 0.05, ** P < 0.01; j * P < 0.05, ** P < 0.01
Dc Generation Medium A, supplied by PromoCell, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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dc medium  (Cellgenix)
90
Cellgenix dc medium
Mechanisms of DC-mediated cross-presentation of LA. a Pathway diagram of DC cross-presenting epitopes. b IFN-γ secretion by ELISPOT assay ( n = 3). c–f Monocytes were obtained with a monocyte attachment medium (C-28051, PromoCell) from PBMCs and then cultured in DC generation <t>medium</t> <t>A</t> <t>(C21050,</t> PromoCell) to harvest immature DCs. Immature DCs were treated with 50 μg/ml peptide twice with an interval of 24 h. Maturation of YL/LA-pulsed DCs was promoted ( n = 3). c Morphological examination. d Presentation ability. e Uptake capacity. f IL-12p70 secretion by ELISA. g LA was mainly taken up by immature DCs through an active transport (37 °C), and others might directly bind to DCs (4 °C). h LA was mainly taken up by phagocytosis (inhibited with cytochalasin D), partly by receptor-mediated internalization (inhibited with heparin) ( n = 3). i Immunofluorescence analysis of co-localization between LA (green, FITC) and different subcellular compartments (red, early endosome, lysosome or endoplasmic reticulum) in immature DCs (blue, DAPI, nuclear) ( n = 3). j The trace of LA was mainly concentrated on MHC-I molecular internalization (inhibited with primaquine), rather than vesicle secretion (inhibited with brefeldin A) and TAP transport (inhibited with ICP47) in DCs ( n = 3). k The combination of LA-FITC and HLA-A2.1 on DC surface (blue, DAPI, nuclear; red, Dil, membrane; green, LA-FITC). l The presentation of LA (963.6) or YL (912.3) on DC surface by UPLC-QTOF-MS. m Immunoblot analysis of total Zap70 proteins and phosphorylated (p-) Zap70 in whole-cell lysates of epitope/DC-CTLs ( n = 3). NC, negative control, water. NP, negative peptide, the peptide (PPGRPSPDN) derived from ECM1 with largest predicted IC 50 of affinity with HLA-A2.1. d–f , h , j P values were obtained from independent-samples t-test; error bars denote standard deviation (SD). d–f Compared with NC, * P < 0.05, ** P < 0.01; h Compared with 37 °C blank, * P < 0.05, ** P < 0.01; j * P < 0.05, ** P < 0.01
Dc Medium, supplied by Cellgenix, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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cellgro dc medium  (Cellgro)
90
Cellgro cellgro dc medium
Mechanisms of DC-mediated cross-presentation of LA. a Pathway diagram of DC cross-presenting epitopes. b IFN-γ secretion by ELISPOT assay ( n = 3). c–f Monocytes were obtained with a monocyte attachment medium (C-28051, PromoCell) from PBMCs and then cultured in DC generation <t>medium</t> <t>A</t> <t>(C21050,</t> PromoCell) to harvest immature DCs. Immature DCs were treated with 50 μg/ml peptide twice with an interval of 24 h. Maturation of YL/LA-pulsed DCs was promoted ( n = 3). c Morphological examination. d Presentation ability. e Uptake capacity. f IL-12p70 secretion by ELISA. g LA was mainly taken up by immature DCs through an active transport (37 °C), and others might directly bind to DCs (4 °C). h LA was mainly taken up by phagocytosis (inhibited with cytochalasin D), partly by receptor-mediated internalization (inhibited with heparin) ( n = 3). i Immunofluorescence analysis of co-localization between LA (green, FITC) and different subcellular compartments (red, early endosome, lysosome or endoplasmic reticulum) in immature DCs (blue, DAPI, nuclear) ( n = 3). j The trace of LA was mainly concentrated on MHC-I molecular internalization (inhibited with primaquine), rather than vesicle secretion (inhibited with brefeldin A) and TAP transport (inhibited with ICP47) in DCs ( n = 3). k The combination of LA-FITC and HLA-A2.1 on DC surface (blue, DAPI, nuclear; red, Dil, membrane; green, LA-FITC). l The presentation of LA (963.6) or YL (912.3) on DC surface by UPLC-QTOF-MS. m Immunoblot analysis of total Zap70 proteins and phosphorylated (p-) Zap70 in whole-cell lysates of epitope/DC-CTLs ( n = 3). NC, negative control, water. NP, negative peptide, the peptide (PPGRPSPDN) derived from ECM1 with largest predicted IC 50 of affinity with HLA-A2.1. d–f , h , j P values were obtained from independent-samples t-test; error bars denote standard deviation (SD). d–f Compared with NC, * P < 0.05, ** P < 0.01; h Compared with 37 °C blank, * P < 0.05, ** P < 0.01; j * P < 0.05, ** P < 0.01
Cellgro Dc Medium, supplied by Cellgro, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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rpmi 1640 medium  (Mediatech)
90
Mediatech rpmi 1640 medium
Mechanisms of DC-mediated cross-presentation of LA. a Pathway diagram of DC cross-presenting epitopes. b IFN-γ secretion by ELISPOT assay ( n = 3). c–f Monocytes were obtained with a monocyte attachment medium (C-28051, PromoCell) from PBMCs and then cultured in DC generation <t>medium</t> <t>A</t> <t>(C21050,</t> PromoCell) to harvest immature DCs. Immature DCs were treated with 50 μg/ml peptide twice with an interval of 24 h. Maturation of YL/LA-pulsed DCs was promoted ( n = 3). c Morphological examination. d Presentation ability. e Uptake capacity. f IL-12p70 secretion by ELISA. g LA was mainly taken up by immature DCs through an active transport (37 °C), and others might directly bind to DCs (4 °C). h LA was mainly taken up by phagocytosis (inhibited with cytochalasin D), partly by receptor-mediated internalization (inhibited with heparin) ( n = 3). i Immunofluorescence analysis of co-localization between LA (green, FITC) and different subcellular compartments (red, early endosome, lysosome or endoplasmic reticulum) in immature DCs (blue, DAPI, nuclear) ( n = 3). j The trace of LA was mainly concentrated on MHC-I molecular internalization (inhibited with primaquine), rather than vesicle secretion (inhibited with brefeldin A) and TAP transport (inhibited with ICP47) in DCs ( n = 3). k The combination of LA-FITC and HLA-A2.1 on DC surface (blue, DAPI, nuclear; red, Dil, membrane; green, LA-FITC). l The presentation of LA (963.6) or YL (912.3) on DC surface by UPLC-QTOF-MS. m Immunoblot analysis of total Zap70 proteins and phosphorylated (p-) Zap70 in whole-cell lysates of epitope/DC-CTLs ( n = 3). NC, negative control, water. NP, negative peptide, the peptide (PPGRPSPDN) derived from ECM1 with largest predicted IC 50 of affinity with HLA-A2.1. d–f , h , j P values were obtained from independent-samples t-test; error bars denote standard deviation (SD). d–f Compared with NC, * P < 0.05, ** P < 0.01; h Compared with 37 °C blank, * P < 0.05, ** P < 0.01; j * P < 0.05, ** P < 0.01
Rpmi 1640 Medium, supplied by Mediatech, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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dendritic cells dc  (PromoCell)
93
PromoCell dendritic cells dc
Mechanisms of DC-mediated cross-presentation of LA. a Pathway diagram of DC cross-presenting epitopes. b IFN-γ secretion by ELISPOT assay ( n = 3). c–f Monocytes were obtained with a monocyte attachment medium (C-28051, PromoCell) from PBMCs and then cultured in DC generation <t>medium</t> <t>A</t> <t>(C21050,</t> PromoCell) to harvest immature DCs. Immature DCs were treated with 50 μg/ml peptide twice with an interval of 24 h. Maturation of YL/LA-pulsed DCs was promoted ( n = 3). c Morphological examination. d Presentation ability. e Uptake capacity. f IL-12p70 secretion by ELISA. g LA was mainly taken up by immature DCs through an active transport (37 °C), and others might directly bind to DCs (4 °C). h LA was mainly taken up by phagocytosis (inhibited with cytochalasin D), partly by receptor-mediated internalization (inhibited with heparin) ( n = 3). i Immunofluorescence analysis of co-localization between LA (green, FITC) and different subcellular compartments (red, early endosome, lysosome or endoplasmic reticulum) in immature DCs (blue, DAPI, nuclear) ( n = 3). j The trace of LA was mainly concentrated on MHC-I molecular internalization (inhibited with primaquine), rather than vesicle secretion (inhibited with brefeldin A) and TAP transport (inhibited with ICP47) in DCs ( n = 3). k The combination of LA-FITC and HLA-A2.1 on DC surface (blue, DAPI, nuclear; red, Dil, membrane; green, LA-FITC). l The presentation of LA (963.6) or YL (912.3) on DC surface by UPLC-QTOF-MS. m Immunoblot analysis of total Zap70 proteins and phosphorylated (p-) Zap70 in whole-cell lysates of epitope/DC-CTLs ( n = 3). NC, negative control, water. NP, negative peptide, the peptide (PPGRPSPDN) derived from ECM1 with largest predicted IC 50 of affinity with HLA-A2.1. d–f , h , j P values were obtained from independent-samples t-test; error bars denote standard deviation (SD). d–f Compared with NC, * P < 0.05, ** P < 0.01; h Compared with 37 °C blank, * P < 0.05, ** P < 0.01; j * P < 0.05, ** P < 0.01
Dendritic Cells Dc, supplied by PromoCell, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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dc medium  (Thermo Fisher)
86
Thermo Fisher dc medium
Mechanisms of DC-mediated cross-presentation of LA. a Pathway diagram of DC cross-presenting epitopes. b IFN-γ secretion by ELISPOT assay ( n = 3). c–f Monocytes were obtained with a monocyte attachment medium (C-28051, PromoCell) from PBMCs and then cultured in DC generation <t>medium</t> <t>A</t> <t>(C21050,</t> PromoCell) to harvest immature DCs. Immature DCs were treated with 50 μg/ml peptide twice with an interval of 24 h. Maturation of YL/LA-pulsed DCs was promoted ( n = 3). c Morphological examination. d Presentation ability. e Uptake capacity. f IL-12p70 secretion by ELISA. g LA was mainly taken up by immature DCs through an active transport (37 °C), and others might directly bind to DCs (4 °C). h LA was mainly taken up by phagocytosis (inhibited with cytochalasin D), partly by receptor-mediated internalization (inhibited with heparin) ( n = 3). i Immunofluorescence analysis of co-localization between LA (green, FITC) and different subcellular compartments (red, early endosome, lysosome or endoplasmic reticulum) in immature DCs (blue, DAPI, nuclear) ( n = 3). j The trace of LA was mainly concentrated on MHC-I molecular internalization (inhibited with primaquine), rather than vesicle secretion (inhibited with brefeldin A) and TAP transport (inhibited with ICP47) in DCs ( n = 3). k The combination of LA-FITC and HLA-A2.1 on DC surface (blue, DAPI, nuclear; red, Dil, membrane; green, LA-FITC). l The presentation of LA (963.6) or YL (912.3) on DC surface by UPLC-QTOF-MS. m Immunoblot analysis of total Zap70 proteins and phosphorylated (p-) Zap70 in whole-cell lysates of epitope/DC-CTLs ( n = 3). NC, negative control, water. NP, negative peptide, the peptide (PPGRPSPDN) derived from ECM1 with largest predicted IC 50 of affinity with HLA-A2.1. d–f , h , j P values were obtained from independent-samples t-test; error bars denote standard deviation (SD). d–f Compared with NC, * P < 0.05, ** P < 0.01; h Compared with 37 °C blank, * P < 0.05, ** P < 0.01; j * P < 0.05, ** P < 0.01
Dc Medium, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Mechanisms of DC-mediated cross-presentation of LA. a Pathway diagram of DC cross-presenting epitopes. b IFN-γ secretion by ELISPOT assay ( n = 3). c–f Monocytes were obtained with a monocyte attachment medium (C-28051, PromoCell) from PBMCs and then cultured in DC generation medium A (C21050, PromoCell) to harvest immature DCs. Immature DCs were treated with 50 μg/ml peptide twice with an interval of 24 h. Maturation of YL/LA-pulsed DCs was promoted ( n = 3). c Morphological examination. d Presentation ability. e Uptake capacity. f IL-12p70 secretion by ELISA. g LA was mainly taken up by immature DCs through an active transport (37 °C), and others might directly bind to DCs (4 °C). h LA was mainly taken up by phagocytosis (inhibited with cytochalasin D), partly by receptor-mediated internalization (inhibited with heparin) ( n = 3). i Immunofluorescence analysis of co-localization between LA (green, FITC) and different subcellular compartments (red, early endosome, lysosome or endoplasmic reticulum) in immature DCs (blue, DAPI, nuclear) ( n = 3). j The trace of LA was mainly concentrated on MHC-I molecular internalization (inhibited with primaquine), rather than vesicle secretion (inhibited with brefeldin A) and TAP transport (inhibited with ICP47) in DCs ( n = 3). k The combination of LA-FITC and HLA-A2.1 on DC surface (blue, DAPI, nuclear; red, Dil, membrane; green, LA-FITC). l The presentation of LA (963.6) or YL (912.3) on DC surface by UPLC-QTOF-MS. m Immunoblot analysis of total Zap70 proteins and phosphorylated (p-) Zap70 in whole-cell lysates of epitope/DC-CTLs ( n = 3). NC, negative control, water. NP, negative peptide, the peptide (PPGRPSPDN) derived from ECM1 with largest predicted IC 50 of affinity with HLA-A2.1. d–f , h , j P values were obtained from independent-samples t-test; error bars denote standard deviation (SD). d–f Compared with NC, * P < 0.05, ** P < 0.01; h Compared with 37 °C blank, * P < 0.05, ** P < 0.01; j * P < 0.05, ** P < 0.01

Journal: Journal of Hematology & Oncology

Article Title: HLA-A2.1-restricted ECM1-derived epitope LA through DC cross-activation priming CD8 + T and NK cells: a novel therapeutic tumour vaccine

doi: 10.1186/s13045-021-01081-7

Figure Lengend Snippet: Mechanisms of DC-mediated cross-presentation of LA. a Pathway diagram of DC cross-presenting epitopes. b IFN-γ secretion by ELISPOT assay ( n = 3). c–f Monocytes were obtained with a monocyte attachment medium (C-28051, PromoCell) from PBMCs and then cultured in DC generation medium A (C21050, PromoCell) to harvest immature DCs. Immature DCs were treated with 50 μg/ml peptide twice with an interval of 24 h. Maturation of YL/LA-pulsed DCs was promoted ( n = 3). c Morphological examination. d Presentation ability. e Uptake capacity. f IL-12p70 secretion by ELISA. g LA was mainly taken up by immature DCs through an active transport (37 °C), and others might directly bind to DCs (4 °C). h LA was mainly taken up by phagocytosis (inhibited with cytochalasin D), partly by receptor-mediated internalization (inhibited with heparin) ( n = 3). i Immunofluorescence analysis of co-localization between LA (green, FITC) and different subcellular compartments (red, early endosome, lysosome or endoplasmic reticulum) in immature DCs (blue, DAPI, nuclear) ( n = 3). j The trace of LA was mainly concentrated on MHC-I molecular internalization (inhibited with primaquine), rather than vesicle secretion (inhibited with brefeldin A) and TAP transport (inhibited with ICP47) in DCs ( n = 3). k The combination of LA-FITC and HLA-A2.1 on DC surface (blue, DAPI, nuclear; red, Dil, membrane; green, LA-FITC). l The presentation of LA (963.6) or YL (912.3) on DC surface by UPLC-QTOF-MS. m Immunoblot analysis of total Zap70 proteins and phosphorylated (p-) Zap70 in whole-cell lysates of epitope/DC-CTLs ( n = 3). NC, negative control, water. NP, negative peptide, the peptide (PPGRPSPDN) derived from ECM1 with largest predicted IC 50 of affinity with HLA-A2.1. d–f , h , j P values were obtained from independent-samples t-test; error bars denote standard deviation (SD). d–f Compared with NC, * P < 0.05, ** P < 0.01; h Compared with 37 °C blank, * P < 0.05, ** P < 0.01; j * P < 0.05, ** P < 0.01

Article Snippet: Fig. 3 Mechanisms of DC-mediated cross-presentation of LA. a Pathway diagram of DC cross-presenting epitopes. b IFN-γ secretion by ELISPOT assay ( n = 3). c–f Monocytes were obtained with a monocyte attachment medium (C-28051, PromoCell) from PBMCs and then cultured in DC generation medium A (C21050, PromoCell) to harvest immature DCs.

Techniques: Enzyme-linked Immunospot, Cell Culture, Enzyme-linked Immunosorbent Assay, Immunofluorescence, Western Blot, Negative Control, Derivative Assay, Standard Deviation